The interfering vectors were microinjected into male pronucleus of the embryos. When cultured in vitro, embryo developmental arrest took place in 2 cell stage. It was obvious that the fluorescence of microinjected embryos became weakened under microscope. 小鼠胚胎原核显微注射干扰载体后进行体外培养,发育多数阻滞在二细胞期,此时荧光显微镜下可观察到明显的荧光减弱现象。
Glucose as the energy source is important for the cell growth, but research indicated that early embryos exposed to high level glucose could experience significant developmental arrest. 葡萄糖是细胞生长重要的能源物质,但研究发现早期胚胎暴露在高葡萄糖环境下会抑制其发育,导致胚胎发育阻滞。